Whatman
Nuclepore Hydrophilic Membrane, 5.0um Pore Size, 13mm Circle, 100/Pk SKU: 10417401

Polycarbonate Track-Etched (PCTE) Membrane Filters - 10417401

The Whatman 10417401 protects sensitive instrumentation and sample integrity by providing reliable particulate removal with minimal analyte adsorption or background interference. Central to its design, quality-controlled manufacturing ensures the filtration medium delivers the retention efficiency and throughput that demanding laboratory protocols require. For this reason, lot-level verification of pore size, flow rate, and physical dimensions ensures that membrane performance remains predictable when scaling from method development to routine analysis.

Material Specifications

• Manufactured with tight pore size control for precise separation of cells and particulates.
• Uniform pore density across the membrane surface ensures representative sampling for environmental and biological particulate analysis.
• Bulk format reduces per-unit packaging waste and cost, appropriate for routine particulate removal and clarification where aseptic handling is not specified.
• Offer a smooth, flat glass-like surface specifically designed for microscopy analysis.
• Low protein binding and biologically inert surface properties make track-etched Polycarbonate compatible with cell biology and cytology workflows.

Maintaining accreditation under ISO 17025 or similar quality frameworks requires documented traceability at every step — the specification compliance of each membrane lot supports your ongoing audit readiness. Low background autofluorescence from polycarbonate membranes keeps your fluorescence microscopy signal-to-noise ratio high, ensuring that the fluorescent labels on your captured particles are what you see — not membrane interference. Secure your research outcomes by partnering with a filtration solution that understands your need for speed, accuracy, and reproducibility.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

N/A

Color

translucent

Diameter

13mm

Extractables

very low

Filtration Area

1.33cm²

Flow Rate

400ml (Water)

Grid (Yes/No)

No

Material

Polycarbonate (PC)

Maximum Operating Pressure

Holder-dependent

Maximum Operating Temperature (°C)

140°C

Membrane Thickness (µm)

6–11um

Pack Count

100

pH Range

2-11

Pore Size (µm)

5

Sterility

Nonsterile

Sterilization

Autoclavable at 121°C for 30 mins

Support Material

None

Wettability

Hydrophilic

• Exosome Isolation - Separates extracellular vesicles from larger cell debris using defined pore sizes, preventing contamination in RNA analysis.
• Erythrocyte Deformability - Mimics capillary dimensions to test red blood cell flexibility and rheology in clinical hemorheology research.
• Chemotaxis Migration - Acts as a precise barrier in Boyden chambers to measure cell migration towards chemoattractants without passive diffusion.
• Asbestos Analysis - Provides a smooth, flat surface for collecting airborne fibers, allowing for direct counting via phase-contrast light microscopy.
• Parasite Detection - Concentrates blood samples to detect microfilariae on a transparent surface, simplifying identification without staining.
• Legionella Recovery - Captures bacteria from hot water samples on a flat plane for direct fluorescent antibody staining and microscope counting.