Whatman
Nuclepore Hydrophilic Membrane, 12.0um Pore Size, 50mm Circle, 100/Pk SKU: 10418514

Polycarbonate Track-Etched (PCTE) Membrane Filters - 10418514

Engineered for high retention efficiency and superior chemical stability, the Whatman 10418514 achieves reliable separation results across a broad range of analytical and preparative applications. Constructed for reliable operation, precision membrane geometry balances retention sharpness with sustainable flow rates, minimizing the pressure buildup that leads to premature filter replacement. Consistent surface properties across the full membrane area support uniform particle capture and reliable results in gravimetric, microscopic, and culture-based analytical workflows.

Material Specifications

• Economical bulk packaging supports laboratories with high consumable throughput where individual sterile wrapping is unnecessary.
• Track-etched Polycarbonate membranes feature perfectly cylindrical pores for absolute fractionation.
• Manufactured with tight pore size control for precise separation of cells and particulates.
• Optically clear construction enables direct on-membrane visualization of captured particles without transfer or additional preparation.
• Low protein binding and biologically inert surface properties make track-etched Polycarbonate compatible with cell biology and cytology workflows.

Microbial enumeration methods require complete retention at the rated pore size and a surface that supports colony growth without inhibition, and the membrane specifications above confirm both requirements for your culture-based workflows. Defined pore geometry gives you a membrane that functions as a precision sieve, capturing particles strictly by size rather than the impaction and diffusion mechanisms that depth filters rely on. Take control of your sample preparation variables and ensure that your data reflects the true nature of your work, rather than the quality of your consumables.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

N/A

Color

translucent

Diameter

50mm

Extractables

very low

Filtration Area

19.63cm²

Flow Rate

30 L (Air)

Grid (Yes/No)

No

Material

Polycarbonate (PC)

Maximum Operating Pressure

Holder-dependent

Maximum Operating Temperature (°C)

140°C

Membrane Thickness (µm)

6–11um

Pack Count

100

pH Range

2-11

Pore Size (µm)

12

Sterility

Nonsterile

Sterilization

Autoclavable at 121°C for 30 mins

Support Material

None

Wettability

Hydrophobic

• Liposome Sizing - Used in extrusion devices to force lipid suspensions through exact pores, creating uniform unilamellar vesicles for drug delivery.
• Parasite Detection - Concentrates blood samples to detect microfilariae on a transparent surface, simplifying identification without staining.
• Chemotaxis Migration - Acts as a precise barrier in Boyden chambers to measure cell migration towards chemoattractants without passive diffusion.
• Exosome Isolation - Separates extracellular vesicles from larger cell debris using defined pore sizes, preventing contamination in RNA analysis.
• Legionella Recovery - Captures bacteria from hot water samples on a flat plane for direct fluorescent antibody staining and microscope counting.
• Erythrocyte Deformability - Mimics capillary dimensions to test red blood cell flexibility and rheology in clinical hemorheology research.