Nuclepore Hydrophilic Membrane, 12.0um Pore Size, 47mm Circle, 100/Pk SKU: 10418512

Polycarbonate Track-Etched (PCTE) Membrane Filters - 10418512

The Whatman 10418512 protects sensitive instrumentation and sample integrity by providing reliable particulate removal with minimal analyte adsorption or background interference. Optimized for laboratory use, a uniform Polycarbonate Track-Etched (PCTE) matrix maintains its rated 12.0 cutoff under the pressures and flow conditions encountered during routine laboratory filtration. From raw polymer through final inspection, the manufacturing process is designed to ensure that every membrane disc meets the performance specifications of its grade designation.

Material Specifications

• Packaged without sterilization for applications where downstream sterility is achieved by other means or is not a process requirement.
• Offer a smooth, flat glass-like surface specifically designed for microscopy analysis.
• Optically clear construction enables direct on-membrane visualization of captured particles without transfer or additional preparation.
• Uniform pore density across the membrane surface ensures representative sampling for environmental and biological particulate analysis.
• Track-etched Polycarbonate membranes feature perfectly cylindrical pores for absolute fractionation.

Microbial enumeration methods require complete retention at the rated pore size and a surface that supports colony growth without inhibition, and the membrane specifications above confirm both requirements for your culture-based workflows. Specifically, low background autofluorescence from polycarbonate membranes keeps your fluorescence microscopy signal-to-noise ratio high, ensuring that the fluorescent labels on your captured particles are what you see — not membrane interference. Take control of your sample preparation variables and ensure that your data reflects the true nature of your work, rather than the quality of your consumables.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

N/A

Color

translucent

Diameter

47mm

Extractables

very low

Filtration Area

17.35cm²

Flow Rate

30 L (Air)

Grid (Yes/No)

No

Material

Polycarbonate (PC)

Maximum Operating Pressure

Holder-dependent

Maximum Operating Temperature (°C)

140°C

Membrane Thickness (µm)

6–11um

Pack Count

100

pH Range

2-11

Pore Size (µm)

12

Sterility

Nonsterile

Sterilization

Autoclavable at 121°C for 30 mins

Support Material

None

Wettability

Hydrophobic

• Oceanographic Sampling - Fractionates plankton populations by size in seawater, ensuring precise separation for biomass and diversity studies.
• Parasite Detection - Concentrates blood samples to detect microfilariae on a transparent surface, simplifying identification without staining.
• Erythrocyte Deformability - Mimics capillary dimensions to test red blood cell flexibility and rheology in clinical hemorheology research.
• Chemotaxis Migration - Acts as a precise barrier in Boyden chambers to measure cell migration towards chemoattractants without passive diffusion.
• Liposome Sizing - Used in extrusion devices to force lipid suspensions through exact pores, creating uniform unilamellar vesicles for drug delivery.
• Exosome Isolation - Separates extracellular vesicles from larger cell debris using defined pore sizes, preventing contamination in RNA analysis.