Polyamide Membrane Circle (NL16), 0.2um Pore Size, 25mm, 100/Pk SKU: 10414006

Nylon Membrane Filters - 10414006

Engineered for high retention efficiency and superior chemical stability, the Whatman 10414006 achieves reliable separation results across a broad range of analytical and preparative applications. Quality-controlled manufacturing ensures the filtration medium delivers the retention efficiency and throughput that demanding laboratory protocols require. Each membrane disc reflects a manufacturing standard where retention characteristics, physical integrity, and dimensional accuracy are verified before release.

Material Specifications

• Naturally hydrophilic and constructed with high tensile strength, Nylon provides reliable performance without pre-wetting in aqueous applications.
• High mechanical strength allows Nylon membranes to withstand elevated differential pressures without structural deformation.
• Broad compatibility with alcohols, aliphatic hydrocarbons, and aromatic solvents extends Nylon filtration into mixed-solvent sample preparation.
• Ideal for filtering aqueous biological solutions and mobile phases.
• Bulk format reduces per-unit packaging waste and cost, appropriate for routine particulate removal and clarification where aseptic handling is not specified.

When your analytical method specifies membrane filtration as a sample preparation step, the retention characteristics and extractable profiles documented above allow you to meet method requirements without additional qualification work. To meet this need, alkaline resistance that other membranes cannot match means you can filter high-pH buffers, basic mobile phases, and caustic wash solutions without the membrane degradation that limits cellulose-based and PES alternatives. Take control of your sample preparation variables and ensure that your data reflects the true nature of your work, rather than the quality of your consumables.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

50-65psi

Color

white

Diameter

25mm

Extractables

low

Filtration Area

4.91cm²

Flow Rate

20ml (Water)

Grid (Yes/No)

No

Material

Nylon

Maximum Operating Pressure

Holder-dependent

Maximum Operating Temperature (°C)

135°C

Membrane Thickness (µm)

110um

Pack Count

100

pH Range

3-14

Pore Size (µm)

0.2

Sterility

Nonsterile

Sterilization

Autoclavable at 121°C for 30 mins

Support Material

None

Wettability

Hydrophilic

• Solid Phase Extraction - Acts as a frit or barrier in custom extraction columns, retaining sorbent beds while allowing solvent to pass through.
• Southern Blot Transfer - Binds nucleic acids strongly during capillary transfer, providing a durable matrix for repeated hybridization cycles.
• Radiolabeled Assays - Retains radiolabeled DNA/RNA fragments efficiently, ensuring accurate quantification in molecular biology experiments.
• Alkaline Filtration - Withstands high-pH solutions during industrial fluid processing, offering durability where other polymers might degrade.
• Atmospheric Testing - Used in dry deposition sampling to capture specific atmospheric nitrates and sulfates for environmental quality studies.
• Northern Blotting - Excellent for RNA transfer applications where high tensile strength is required for multiple rounds of probe stripping.