Whatman
Nylon Membrane Circle, 0.45um Pore Size, 25mm, 100/Pk SKU: 7404-002

Nylon Membrane Filters - 7404-002

Engineered for high retention efficiency and superior chemical stability, the Whatman 7404-002 achieves reliable separation results across a broad range of analytical and preparative applications. Quality-controlled manufacturing ensures the filtration medium delivers the retention efficiency and throughput that demanding laboratory protocols require. In combination, precision manufacturing ensures that each membrane disc delivers the rated retention, flow rate, and surface consistency that analytical and microbiological methods require.

Material Specifications

• Naturally hydrophilic and constructed with high tensile strength, Nylon provides reliable performance without pre-wetting in aqueous applications.
• Economical bulk packaging supports laboratories with high consumable throughput where individual sterile wrapping is unnecessary.
• Ideal for filtering aqueous biological solutions and mobile phases.
• Alkaline-resistant and compatible with a broad range of aqueous buffers used in pharmaceutical and biotechnology laboratories.
• Flexible and durable, eliminating the need for wetting agents that could contaminate samples.

Transitioning from qualitative screening to quantitative gravimetric analysis requires a membrane with documented tare weight consistency, and the manufacturing tolerances above ensure that your blank values remain within acceptable limits. For exactly this reason, the natural wettability of nylon allows you to begin filtration immediately upon sample contact, eliminating the bubble formation and flow interruption that hydrophobic membranes introduce when processing aqueous samples without pre-treatment. Take control of your sample preparation variables and ensure that your data reflects the true nature of your work, rather than the quality of your consumables.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

34-42psi

Color

white

Diameter

25mm

Extractables

low

Filtration Area

4.91cm²

Flow Rate

> 60ml (Water)

Grid (Yes/No)

No

Material

Nylon

Maximum Operating Pressure

Holder-dependent

Maximum Operating Temperature (°C)

135°C

Membrane Thickness (µm)

150-187um

Pack Count

100

pH Range

4-9

Pore Size (µm)

0.45

Sterility

Nonsterile

Sterilization

Autoclavable at 121°C for 30 mins

Support Material

None

Wettability

Hydrophilic

• Southern Blot Transfer - Binds nucleic acids strongly during capillary transfer, providing a durable matrix for repeated hybridization cycles.
• Atmospheric Testing - Used in dry deposition sampling to capture specific atmospheric nitrates and sulfates for environmental quality studies.
• Alkaline Filtration - Withstands high-pH solutions during industrial fluid processing, offering durability where other polymers might degrade.
• Northern Blotting - Excellent for RNA transfer applications where high tensile strength is required for multiple rounds of probe stripping.
• Radiolabeled Assays - Retains radiolabeled DNA/RNA fragments efficiently, ensuring accurate quantification in molecular biology experiments.
• Solid Phase Extraction - Acts as a frit or barrier in custom extraction columns, retaining sorbent beds while allowing solvent to pass through.