AE98 5.0um 50mm 100/Pk SKU: 10400214

Cellulose Nitrate (CN) Membrane Filters - 10400214

The Whatman 10400214 protects sensitive instrumentation and sample integrity by providing reliable particulate removal with minimal analyte adsorption or background interference. Furthermore, precision-manufactured Hydrophilic membrane with a verified 5.0 pore structure delivers consistent retention across the full operating range. From raw polymer through final inspection, the manufacturing process is designed to ensure that every membrane disc meets the performance specifications of its grade designation.

Material Specifications

• High protein binding capacity makes cellulose nitrate the preferred membrane for Western blotting, dot blotting, and nucleic acid immobilization techniques that rely on efficient surface adsorption.
• Surface retention characteristics allow captured particles and microorganisms to remain on the membrane face, supporting direct microscopic examination and physical recovery of collected material.
• Naturally hydrophilic and compatible with aqueous solutions, cellulose nitrate delivers high flow rates and rapid wet-out without requiring pre-conditioning or surfactant treatment.
• Non-sterile configuration is suited for analytical sample preparation, mobile phase filtration, and other workflows where pre-sterilization is not required.
• Cellulose nitrate membranes feature a narrow pore size distribution and low extractable levels, providing the controlled retention characteristics required for gravimetric and particulate analysis.

Air monitoring and stack emissions sampling demand a filter medium that withstands the thermal and chemical conditions at your collection point — the material properties described above define the operating envelope for your application. In particular, low extractable levels in cellulose nitrate membranes mean your blank measurements stay minimal, giving you the confidence that weight changes in gravimetric analysis reflect actual sample content rather than membrane artifacts. Take control of your sample preparation variables and ensure that your data reflects the true nature of your work, rather than the quality of your consumables.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

6-8psi

Color

white

Diameter

50mm

Extractables

low

Filtration Area

19.63cm²

Flow Rate

350ml (Water)

Grid (Yes/No)

No

Material

Cellulose Nitrate (CN)

Maximum Operating Pressure

Holder-dependent

Maximum Operating Temperature (°C)

80°C

Membrane Thickness (µm)

105–140um

Pack Count

100

pH Range

4-8

Pore Size (µm)

5

Sterility

Nonsterile

Sterilization

Autoclavable at 121°C for 30 mins

Support Material

None

Wettability

Hydrophilic

• Water Quality Testing - Narrow pore distribution retains indicator organisms consistently for regulatory compliance in environmental laboratories.
• Colony Enumeration - Gridded surface variants facilitate accurate plate counting of captured microorganisms under standard magnification.
• Aerosol Analysis - Low tare weight and acid digestibility enable chemical characterization of airborne particulates from air sampling.
• Gravimetric Particulate Analysis - Dissolves in organic solvents for complete particle recovery and accurate weight-based quantification.
• Routine Sample Prep - Uniform microporous matrix provides consistent flow rates and reliable particle retention for general aqueous solutions.
• Bioburden Monitoring - Surface retention keeps captured organisms accessible for direct incubation and enumeration without transfer steps.