Whatman
NC45 0.45um 47mm 100/Pk SKU: 10401112

Cellulose Nitrate (CN) Membrane Filters - 10401112

Engineered for high retention efficiency and superior chemical stability, the Whatman 10401112 achieves reliable separation results across a broad range of analytical and preparative applications. Moreover, reproducible separation characteristics define the membrane structure, protecting downstream instruments and preserving sample integrity throughout the filtration process. In effect, precision manufacturing ensures that each membrane disc delivers the rated retention, flow rate, and surface consistency that analytical and microbiological methods require.

Material Specifications

• High protein binding capacity makes cellulose nitrate the preferred membrane for Western blotting, dot blotting, and nucleic acid immobilization techniques that rely on efficient surface adsorption.
• Cellulose nitrate membranes feature a narrow pore size distribution and low extractable levels, providing the controlled retention characteristics required for gravimetric and particulate analysis.
• Surface retention characteristics allow captured particles and microorganisms to remain on the membrane face, supporting direct microscopic examination and physical recovery of collected material.
• Autoclavable at 121°C without loss of integrity, cellulose nitrate membranes maintain their rated pore structure and dimensional stability through repeated sterilization cycles.
• Dissolves completely in specific organic solvents, enabling full recovery of captured particles from the membrane surface for downstream gravimetric, chemical, or spectroscopic analysis.

Transitioning from qualitative screening to quantitative gravimetric analysis requires a membrane with documented tare weight consistency, and the manufacturing tolerances above ensure that your blank values remain within acceptable limits. The narrow pore size distribution of cellulose nitrate membranes ensures your retention data is consistent across batches, reducing the inter-sample variability that complicates quantitative particulate and microbiological analyses. Take control of your sample preparation variables and ensure that your data reflects the true nature of your work, rather than the quality of your consumables.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

40.6psi

Color

white

Diameter

47mm

Extractables

low

Filtration Area

17.35cm²

Flow Rate

60ml (Water)

Grid (Yes/No)

No

Material

Cellulose Nitrate (CN)

Maximum Operating Pressure

Holder-dependent

Maximum Operating Temperature (°C)

80°C

Membrane Thickness (µm)

105–140um

Pack Count

100

pH Range

4-8

Pore Size (µm)

0.45

Sterility

Nonsterile

Sterilization

Autoclavable at 121°C for 30 mins

Support Material

None

Wettability

Hydrophilic

• Biochemical Assays - High protein-binding capacity effectively immobilizes macromolecules, supporting lateral flow and diagnostic testing.
• Routine Sample Prep - Uniform microporous matrix provides consistent flow rates and reliable particle retention for general aqueous solutions.
• Gravimetric Particulate Analysis - Dissolves in organic solvents for complete particle recovery and accurate weight-based quantification.
• Aerosol Analysis - Low tare weight and acid digestibility enable chemical characterization of airborne particulates from air sampling.
• Bioburden Monitoring - Surface retention keeps captured organisms accessible for direct incubation and enumeration without transfer steps.
• Water Quality Testing - Narrow pore distribution retains indicator organisms consistently for regulatory compliance in environmental laboratories.