NC45 0.45um 25mm 100/Pk SKU: 10401106

Cellulose Nitrate (CN) Membrane Filters - 10401106

Designed to deliver reproducibility and confidence across critical sample types, the Whatman 10401106 provides the filtration consistency that method validation demands. Central to its design, quality-controlled manufacturing ensures the filtration medium delivers the retention efficiency and throughput that demanding laboratory protocols require. In sum, precision manufacturing ensures that each membrane disc delivers the rated retention, flow rate, and surface consistency that analytical and microbiological methods require.

Material Specifications

• High protein binding capacity makes cellulose nitrate the preferred membrane for Western blotting, dot blotting, and nucleic acid immobilization techniques that rely on efficient surface adsorption.
• Surface retention characteristics allow captured particles and microorganisms to remain on the membrane face, supporting direct microscopic examination and physical recovery of collected material.
• Autoclavable at 121°C without loss of integrity, cellulose nitrate membranes maintain their rated pore structure and dimensional stability through repeated sterilization cycles.
• Naturally hydrophilic and compatible with aqueous solutions, cellulose nitrate delivers high flow rates and rapid wet-out without requiring pre-conditioning or surfactant treatment.
• Economical bulk packaging supports laboratories with high consumable throughput where individual sterile wrapping is unnecessary.

Multi-site studies where different laboratories must produce comparable data benefit from membranes manufactured to tight specifications, giving you confidence that site-to-site variability is not introduced by the filtration step. In these cases, surface retention on cellulose nitrate keeps captured particles and microorganisms on the membrane face where they remain accessible for direct microscopic examination, colony counting, and physical recovery without sectioning or dissolution. Elevate the quality of your analytical data today by standardizing on a filtration product built to meet the exact specifications of your applications.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

40.6psi

Color

white

Diameter

25mm

Extractables

low

Filtration Area

4.91cm²

Flow Rate

60ml (Water)

Grid (Yes/No)

No

Material

Cellulose Nitrate (CN)

Maximum Operating Pressure

Holder-dependent

Maximum Operating Temperature (°C)

80°C

Membrane Thickness (µm)

105–140um

Pack Count

100

pH Range

4-8

Pore Size (µm)

0.45

Sterility

Nonsterile

Sterilization

Autoclavable at 121°C for 30 mins

Support Material

None

Wettability

Hydrophilic

• Environmental Compliance - Documented retention characteristics at standard pore ratings support reproducibility in regulated testing programs.
• Aerosol Analysis - Low tare weight and acid digestibility enable chemical characterization of airborne particulates from air sampling.
• Colony Enumeration - Gridded surface variants facilitate accurate plate counting of captured microorganisms under standard magnification.
• Microscopy Examination - Particles remain on the membrane surface for direct visual inspection, supporting identification and classification.
• Routine Sample Prep - Uniform microporous matrix provides consistent flow rates and reliable particle retention for general aqueous solutions.
• Gravimetric Particulate Analysis - Dissolves in organic solvents for complete particle recovery and accurate weight-based quantification.