CA Membrane Circle (OE 67), 0.45um Pore Size, 47mm Circle (100 pcs) SKU: 10404012

Cellulose Acetate (CA) Membrane Filters - 10404012

Designed to deliver reproducibility and confidence across critical sample types, the Whatman 10404012 provides the filtration consistency that method validation demands. Precision-manufactured Hydrophilic membrane with a verified 0.45 pore structure delivers consistent retention across the full operating range. Controlled production conditions produce membranes with documented pore size distribution and thickness tolerances, supporting method reproducibility across multiple filter lots.

Material Specifications

• Distinguished by their very low protein binding, making them ideal for protein recovery.
• Offer improved chemical resistance to low molecular weight alcohols compared to MCE.
• Naturally hydrophilic and compatible with aqueous biological solutions without pre-wetting or surfactant treatment.
• Bulk packaging format provides cost-effective access for high-volume laboratories performing non-critical filtration and sample preparation.
• Particularly well suited for electrophoresis and immunoassay support applications where predictable binding behavior is essential.

Scaling your method from development to routine production requires membrane consistency at every lot — the documented specifications of these membranes provide the reproducibility needed to validate the filtration step once. For applications like these, the thermal tolerance of cellulose acetate allows autoclaving for applications where you need a sterilized membrane seated in your holder before introducing the sample, simplifying aseptic technique in your workflow. Take control of your sample preparation variables and ensure that your data reflects the true nature of your work, rather than the quality of your consumables.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

35.0psi

Color

white

Diameter

47mm

Extractables

low

Filtration Area

17.35cm²

Flow Rate

55ml (Water)

Grid (Yes/No)

No

Material

Cellulose Acetate (CA)

Maximum Operating Pressure

Holder-dependent

Maximum Operating Temperature (°C)

180°C

Membrane Thickness (µm)

135um

Pack Count

100

pH Range

4-8

Pore Size (µm)

0.45

Sterility

Nonsterile

Sterilization

Autoclavable at 121°C for 30 mins

Support Material

None

Wettability

Hydrophilic

• Isotope Studies - Used in binding studies where the membrane itself must not interact with the radioactive ligand or the receptor protein.
• Enzyme Sterilization - Preserves enzymatic activity during cold sterilization, preventing the denaturation often caused by surface binding.
• Wine Stability Testing - Filters wine samples to check for colloidal stability, ensuring the product will not precipitate during shelf storage.
• Nutrient Analysis - Clarifies water for nitrate/phosphate testing without leaching nitrogenous material that could bias the results.
• Tissue Culture Media - Sterilizes heat-labile media components with minimal hold-up volume, ensuring expensive additives are not wasted.
• Biological Fluid Prep - Clarifies urine or cerebrospinal fluid for clinical chemistry without removing the low-abundance proteins of interest.