Whatman
Nuclepore Hydrophilic Membrane, 5.0um Pore Size, 50mm Circle, 100/Pk SKU: 10417414

Polycarbonate Track-Etched (PCTE) Membrane Filters - 10417414

The Whatman 10417414 combines a controlled pore size distribution with low extractable levels, ensuring the data accuracy that sensitive analytical methods require. Reproducible separation characteristics define the membrane structure, protecting downstream instruments and preserving sample integrity throughout the filtration process. Controlled production conditions produce membranes with documented pore size distribution and thickness tolerances, supporting method reproducibility across multiple filter lots.

Material Specifications

• Low protein binding and biologically inert surface properties make track-etched Polycarbonate compatible with cell biology and cytology workflows.
• Track-etched Polycarbonate membranes feature perfectly cylindrical pores for absolute fractionation.
• Uniform pore density across the membrane surface ensures representative sampling for environmental and biological particulate analysis.
• Bulk packaging format provides cost-effective access for high-volume laboratories performing non-critical filtration and sample preparation.
• Optically clear construction enables direct on-membrane visualization of captured particles without transfer or additional preparation.

Multi-site studies where different laboratories must produce comparable data benefit from membranes manufactured to tight specifications, giving you confidence that site-to-site variability is not introduced by the filtration step. In response, captured particles sit in a single focal plane on the flat polycarbonate surface, making your microscopic examination faster and particle counts more accurate than with depth filters that trap particles at multiple levels. Elevate the quality of your analytical data today by standardizing on a filtration product built to meet the exact specifications of your applications.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

N/A

Color

translucent

Diameter

50mm

Extractables

very low

Filtration Area

19.63cm²

Flow Rate

400ml (Water)

Grid (Yes/No)

No

Material

Polycarbonate (PC)

Maximum Operating Pressure

Holder-dependent

Maximum Operating Temperature (°C)

140°C

Membrane Thickness (µm)

6–11um

Pack Count

100

pH Range

2-11

Pore Size (µm)

5

Sterility

Nonsterile

Sterilization

Autoclavable at 121°C for 30 mins

Support Material

None

Wettability

Hydrophilic

• Exosome Isolation - Separates extracellular vesicles from larger cell debris using defined pore sizes, preventing contamination in RNA analysis.
• Legionella Recovery - Captures bacteria from hot water samples on a flat plane for direct fluorescent antibody staining and microscope counting.
• Chemotaxis Migration - Acts as a precise barrier in Boyden chambers to measure cell migration towards chemoattractants without passive diffusion.
• Liposome Sizing - Used in extrusion devices to force lipid suspensions through exact pores, creating uniform unilamellar vesicles for drug delivery.
• Asbestos Analysis - Provides a smooth, flat surface for collecting airborne fibers, allowing for direct counting via phase-contrast light microscopy.
• Parasite Detection - Concentrates blood samples to detect microfilariae on a transparent surface, simplifying identification without staining.