Nuclepore Hydrophilic Membrane, 0.4um Pore Size, 13mm Circle, 100/Pk SKU: 10417101

Polycarbonate Track-Etched (PCTE) Membrane Filters - 10417101

Engineered for high retention efficiency and superior chemical stability, the Whatman 10417101 achieves reliable separation results across a broad range of analytical and preparative applications. At its core, verified 0.4 pore distribution across the Hydrophilic membrane surface guarantees batch-to-batch consistency in particle capture and analyte passage. Working in concert, precision manufacturing ensures that each membrane disc delivers the rated retention, flow rate, and surface consistency that analytical and microbiological methods require.

Material Specifications

• Track-etched Polycarbonate membranes feature perfectly cylindrical pores for absolute fractionation.
• Supplied in bulk non-sterile packaging, offering an economical option for routine clarification and general laboratory filtration tasks.
• Manufactured with tight pore size control for precise separation of cells and particulates.
• Offer a smooth, flat glass-like surface specifically designed for microscopy analysis.
• Uniform pore density across the membrane surface ensures representative sampling for environmental and biological particulate analysis.

Whether your method calls for gravimetric analysis, particle visualization, or microbial enumeration, the pore consistency and surface uniformity documented above ensure that your results reflect the sample — not the filter. For this purpose, polycarbonate's optical clarity when wet allows direct on-membrane visualization without clearing or staining steps, streamlining your microscopy workflow and reducing the sample preparation time between filtration and analysis. Take control of your sample preparation variables and ensure that your data reflects the true nature of your work, rather than the quality of your consumables.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

N/A

Color

translucent

Diameter

13mm

Extractables

very low

Filtration Area

1.33cm²

Flow Rate

33ml (Water)

Grid (Yes/No)

No

Material

Polycarbonate (PC)

Maximum Operating Pressure

Holder-dependent

Maximum Operating Temperature (°C)

140°C

Membrane Thickness (µm)

6–11um

Pack Count

100

pH Range

2-11

Pore Size (µm)

0.4

Sterility

Nonsterile

Sterilization

Autoclavable at 121°C for 30 mins

Support Material

None

Wettability

Hydrophilic

• Legionella Recovery - Captures bacteria from hot water samples on a flat plane for direct fluorescent antibody staining and microscope counting.
• Chemotaxis Migration - Acts as a precise barrier in Boyden chambers to measure cell migration towards chemoattractants without passive diffusion.
• Oceanographic Sampling - Fractionates plankton populations by size in seawater, ensuring precise separation for biomass and diversity studies.
• Exosome Isolation - Separates extracellular vesicles from larger cell debris using defined pore sizes, preventing contamination in RNA analysis.
• Liposome Sizing - Used in extrusion devices to force lipid suspensions through exact pores, creating uniform unilamellar vesicles for drug delivery.
• Parasite Detection - Concentrates blood samples to detect microfilariae on a transparent surface, simplifying identification without staining.