Nylon Membrane Circle, 0.8um Pore Size, 47mm, 100/Pk SKU: 7408-004

Nylon Membrane Filters - 7408-004

Designed to deliver reproducibility and confidence across critical sample types, the Whatman 7408-004 provides the filtration consistency that method validation demands. Reproducible separation characteristics define the membrane structure, protecting downstream instruments and preserving sample integrity throughout the filtration process. With this in mind, lot-level verification of pore size, flow rate, and physical dimensions ensures that membrane performance remains predictable when scaling from method development to routine analysis.

Material Specifications

• Alkaline-resistant and compatible with a broad range of aqueous buffers used in pharmaceutical and biotechnology laboratories.
• Flexible and durable, eliminating the need for wetting agents that could contaminate samples.
• Naturally hydrophilic and constructed with high tensile strength, Nylon provides reliable performance without pre-wetting in aqueous applications.
• Packaged without sterilization for applications where downstream sterility is achieved by other means or is not a process requirement.
• Ideal for filtering aqueous biological solutions and mobile phases.

Maintaining accreditation under ISO 17025 or similar quality frameworks requires documented traceability at every step — the specification compliance of each membrane lot supports your ongoing audit readiness. Nylon's compatibility with both aqueous and moderate organic solvent systems lets you standardize on one membrane type across HPLC sample prep workflows that alternate between reversed-phase and normal-phase mobile phases. Secure your research outcomes by partnering with a filtration solution that understands your need for speed, accuracy, and reproducibility.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

> 13psi

Color

white

Diameter

47mm

Extractables

low

Filtration Area

17.35cm²

Flow Rate

> 180ml (Water)

Grid (Yes/No)

No

Material

Nylon

Maximum Operating Pressure

Holder-dependent

Maximum Operating Temperature (°C)

135°C

Membrane Thickness (µm)

137-200um

Pack Count

100

pH Range

3-14

Pore Size (µm)

0.8

Sterility

Nonsterile

Sterilization

Autoclavable at 121°C for 30 mins

Support Material

None

Wettability

Hydrophilic

• Southern Blot Transfer - Binds nucleic acids strongly during capillary transfer, providing a durable matrix for repeated hybridization cycles.
• Atmospheric Testing - Used in dry deposition sampling to capture specific atmospheric nitrates and sulfates for environmental quality studies.
• Alkaline Filtration - Withstands high-pH solutions during industrial fluid processing, offering durability where other polymers might degrade.
• Solvent Mobilization - Clarifies organic mobile phases in glass vacuum holders, preventing particulate damage to sensitive HPLC pump seals.
• Northern Blotting - Excellent for RNA transfer applications where high tensile strength is required for multiple rounds of probe stripping.
• Radiolabeled Assays - Retains radiolabeled DNA/RNA fragments efficiently, ensuring accurate quantification in molecular biology experiments.