Nylon Membrane Circle, 0.45um Pore Size, 90mm, 50/Pk SKU: 7404-009

Nylon Membrane Filters - 7404-009

Engineered for high retention efficiency and superior chemical stability, the Whatman 7404-009 achieves reliable separation results across a broad range of analytical and preparative applications. Constructed for reliable operation, reproducible separation characteristics define the membrane structure, protecting downstream instruments and preserving sample integrity throughout the filtration process. As designed, lot-level verification of pore size, flow rate, and physical dimensions ensures that membrane performance remains predictable when scaling from method development to routine analysis.

Material Specifications

• Alkaline-resistant and compatible with a broad range of aqueous buffers used in pharmaceutical and biotechnology laboratories.
• Flexible and durable, eliminating the need for wetting agents that could contaminate samples.
• Naturally hydrophilic and constructed with high tensile strength, Nylon provides reliable performance without pre-wetting in aqueous applications.
• High mechanical strength allows Nylon membranes to withstand elevated differential pressures without structural deformation.
• Ideal for filtering aqueous biological solutions and mobile phases.

If your protocol requires direct on-membrane analysis by microscopy or culture, the surface characteristics and dimensional tolerances described above give you a substrate that performs consistently from disc to disc. In practice, alkaline resistance that other membranes cannot match means you can filter high-pH buffers, basic mobile phases, and caustic wash solutions without the membrane degradation that limits cellulose-based and PES alternatives. Make the decision for consistency and reliability, ensuring that your laboratory maintains its reputation for high-quality, error-free output.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

34-42psi

Color

white

Diameter

90mm

Extractables

low

Filtration Area

63.62cm²

Flow Rate

> 60ml (Water)

Grid (Yes/No)

No

Material

Nylon

Maximum Operating Pressure

Holder-dependent

Maximum Operating Temperature (°C)

135°C

Membrane Thickness (µm)

150-187um

Pack Count

50

pH Range

3-14

Pore Size (µm)

0.45

Sterility

Nonsterile

Sterilization

Autoclavable at 121°C for 30 mins

Support Material

None

Wettability

Hydrophilic

• Radiolabeled Assays - Retains radiolabeled DNA/RNA fragments efficiently, ensuring accurate quantification in molecular biology experiments.
• Northern Blotting - Excellent for RNA transfer applications where high tensile strength is required for multiple rounds of probe stripping.
• Gene Mapping Support - Provides a stable, charge-modified surface for DNA immobilization, facilitating precise gene mapping and analysis steps.
• Atmospheric Testing - Used in dry deposition sampling to capture specific atmospheric nitrates and sulfates for environmental quality studies.
• Southern Blot Transfer - Binds nucleic acids strongly during capillary transfer, providing a durable matrix for repeated hybridization cycles.
• Alkaline Filtration - Withstands high-pH solutions during industrial fluid processing, offering durability where other polymers might degrade.