Whatman
Nylon Membrane Circle, 0.45um Pore Size, 47mm, 100/Pk SKU: 7404-004

Nylon Membrane Filters - 7404-004

Designed to deliver reproducibility and confidence across critical sample types, the Whatman 7404-004 provides the filtration consistency that method validation demands. At its core, a uniform Nylon matrix maintains its rated 0.45 cutoff under the pressures and flow conditions encountered during routine laboratory filtration. By design, from raw polymer through final inspection, the manufacturing process is designed to ensure that every membrane disc meets the performance specifications of its grade designation.

Material Specifications

• Broad compatibility with alcohols, aliphatic hydrocarbons, and aromatic solvents extends Nylon filtration into mixed-solvent sample preparation.
• High mechanical strength allows Nylon membranes to withstand elevated differential pressures without structural deformation.
• Naturally hydrophilic and constructed with high tensile strength, Nylon provides reliable performance without pre-wetting in aqueous applications.
• Bulk packaging format provides cost-effective access for high-volume laboratories performing non-critical filtration and sample preparation.
• Alkaline-resistant and compatible with a broad range of aqueous buffers used in pharmaceutical and biotechnology laboratories.

Maintaining accreditation under ISO 17025 or similar quality frameworks requires documented traceability at every step — the specification compliance of each membrane lot supports your ongoing audit readiness. In these cases, alkaline resistance that other membranes cannot match means you can filter high-pH buffers, basic mobile phases, and caustic wash solutions without the membrane degradation that limits cellulose-based and PES alternatives. Elevate the quality of your analytical data today by standardizing on a filtration product built to meet the exact specifications of your applications.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

34-42psi

Color

white

Diameter

47mm

Extractables

low

Filtration Area

17.35cm²

Flow Rate

> 60ml (Water)

Grid (Yes/No)

No

Material

Nylon

Maximum Operating Pressure

Holder-dependent

Maximum Operating Temperature (°C)

135°C

Membrane Thickness (µm)

150-187um

Pack Count

100

pH Range

3-14

Pore Size (µm)

0.45

Sterility

Nonsterile

Sterilization

Autoclavable at 121°C for 30 mins

Support Material

None

Wettability

Hydrophilic

• Solid Phase Extraction - Acts as a frit or barrier in custom extraction columns, retaining sorbent beds while allowing solvent to pass through.
• Southern Blot Transfer - Binds nucleic acids strongly during capillary transfer, providing a durable matrix for repeated hybridization cycles.
• Northern Blotting - Excellent for RNA transfer applications where high tensile strength is required for multiple rounds of probe stripping.
• Solvent Mobilization - Clarifies organic mobile phases in glass vacuum holders, preventing particulate damage to sensitive HPLC pump seals.
• Gene Mapping Support - Provides a stable, charge-modified surface for DNA immobilization, facilitating precise gene mapping and analysis steps.
• Atmospheric Testing - Used in dry deposition sampling to capture specific atmospheric nitrates and sulfates for environmental quality studies.