Nylon Membrane Circle, 0.45um Pore Size, 13mm, 100/Pk SKU: 7404-001

Nylon Membrane Filters - 7404-001

The Whatman 7404-001 protects sensitive instrumentation and sample integrity by providing reliable particulate removal with minimal analyte adsorption or background interference. Reproducible separation characteristics define the membrane structure, protecting downstream instruments and preserving sample integrity throughout the filtration process. Consistent surface properties across the full membrane area support uniform particle capture and reliable results in gravimetric, microscopic, and culture-based analytical workflows.

Material Specifications

• Naturally hydrophilic and constructed with high tensile strength, Nylon provides reliable performance without pre-wetting in aqueous applications.
• Flexible and durable, eliminating the need for wetting agents that could contaminate samples.
• Broad compatibility with alcohols, aliphatic hydrocarbons, and aromatic solvents extends Nylon filtration into mixed-solvent sample preparation.
• Ideal for filtering aqueous biological solutions and mobile phases.
• High mechanical strength allows Nylon membranes to withstand elevated differential pressures without structural deformation.

Microbial enumeration methods require complete retention at the rated pore size and a surface that supports colony growth without inhibition, and the membrane specifications above confirm both requirements for your culture-based workflows. The natural wettability of nylon allows you to begin filtration immediately upon sample contact, eliminating the bubble formation and flow interruption that hydrophobic membranes introduce when processing aqueous samples without pre-treatment. Secure your research outcomes by partnering with a filtration solution that understands your need for speed, accuracy, and reproducibility.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

34-42psi

Color

white

Diameter

13mm

Extractables

low

Filtration Area

1.33cm²

Flow Rate

> 60ml (Water)

Grid (Yes/No)

No

Material

Nylon

Maximum Operating Pressure

Holder-dependent

Maximum Operating Temperature (°C)

135°C

Membrane Thickness (µm)

150-187um

Pack Count

100

pH Range

3-14

Pore Size (µm)

0.45

Sterility

Nonsterile

Sterilization

Autoclavable at 121°C for 30 mins

Support Material

None

Wettability

Hydrophilic

• Radiolabeled Assays - Retains radiolabeled DNA/RNA fragments efficiently, ensuring accurate quantification in molecular biology experiments.
• Southern Blot Transfer - Binds nucleic acids strongly during capillary transfer, providing a durable matrix for repeated hybridization cycles.
• Alkaline Filtration - Withstands high-pH solutions during industrial fluid processing, offering durability where other polymers might degrade.
• Atmospheric Testing - Used in dry deposition sampling to capture specific atmospheric nitrates and sulfates for environmental quality studies.
• Gene Mapping Support - Provides a stable, charge-modified surface for DNA immobilization, facilitating precise gene mapping and analysis steps.
• Solid Phase Extraction - Acts as a frit or barrier in custom extraction columns, retaining sorbent beds while allowing solvent to pass through.