Whatman
TURBOBL REFI NY-SPC 150x150mm SKU: 10416314

Nytran Nylon Blotting Membranes - 10416314

Nucleic acid transfer and hybridization experiments demand a nylon membrane that combines very high binding capacity with the mechanical durability required for multiple rounds of stripping and reprobing. Reinforced nylon construction provides the tear resistance and handling durability needed for multiple probe stripping and rehybridization cycles without membrane degradation or signal loss. Quality-controlled manufacturing ensures the membrane consistency that demanding blotting applications require for reproducible, defensible results.

Membrane Specifications

• Engineered for Southern, Northern, colony lift, and dot blot applications, Nytran membranes support both radioactive and nonradioactive detection systems with the sensitivity needed for low-copy-number target sequences.
• Nytran nylon blotting membranes are cast uniformly on both sides of a reinforced support matrix, providing the symmetrical structure that prevents curling and ensures flat, even contact during capillary and electrophoretic transfer.
• Nytran membranes combine high nucleic acid binding capacity with mechanical durability that withstands multiple rounds of stripping and reprobing, making them the preferred substrate for multi-probe hybridization experiments.
• High mechanical strength withstands multiple rounds of probe stripping and rehybridization without membrane degradation or loss of bound target signal.
• Compatible with both isotopic and nonisotopic detection systems including radiolabeled probes, DIG-labeled probes, and biotin-streptavidin detection.

When your research timeline depends on getting defensible blotting data without repeated optimization cycles, the transfer efficiency and signal clarity built into this membrane help you move from experiment to answer with confidence. Compatibility with both radioactive and nonradioactive detection systems provides the analytical flexibility to transition between probe labeling strategies without changing the underlying membrane platform. For questions about chemical compatibility, operating limits, or equivalent product cross-references, Tisch Scientific's filtration specialists can provide the technical detail your method documentation requires.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Dimensions

150mm x 150mm

Material

Nylon

Sterility

Nonsterile

Wettability

Hydrophilic

• Northern Blotting — Transfer and probe hybridization of total or mRNA for tissue-specific gene expression profiling and transcript characterization.
• Nucleic Acid Library Screening — High-throughput identification of target clones from genomic and cDNA libraries using labeled probes.
• Dot and Slot Blotting — Rapid nucleic acid quantitation and sequence-specific screening by direct sample application to charged nylon.
• Restriction Fragment Length Analysis — Detection of polymorphic DNA fragments by Southern hybridization for genotyping and linkage mapping studies.
• Southern Blotting — Alkaline capillary transfer and hybridization of genomic DNA restriction fragments for gene detection and structural analysis.
• Colony and Plaque Lifts — High-density library screening via in situ transfer and hybridization of bacterial colonies or bacteriophage plaques.