Sterile MicroPlus -21 STL Membrane, w/ 3.1mm Black Grid for Membrane-Butler, 0.45um, 50mm SKU: 10407714

Cellulose Nitrate (CN) Membrane Filters - 10407714

Designed to deliver reproducibility and confidence across critical sample types, the Whatman 10407714 provides the filtration consistency that method validation demands. A uniform Cellulose Nitrate (CN) matrix maintains its rated 0.45 cutoff under the pressures and flow conditions encountered during routine laboratory filtration. With this in mind, from raw polymer through final inspection, the manufacturing process is designed to ensure that every membrane disc meets the performance specifications of its grade designation.

Material Specifications

• Autoclavable at 121°C without loss of integrity, cellulose nitrate membranes maintain their rated pore structure and dimensional stability through repeated sterilization cycles.
• High protein binding capacity makes cellulose nitrate the preferred membrane for Western blotting, dot blotting, and nucleic acid immobilization techniques that rely on efficient surface adsorption.
• Surface retention characteristics allow captured particles and microorganisms to remain on the membrane face, supporting direct microscopic examination and physical recovery of collected material.
• Validated Gamma sterilization process delivers documented SAL (Sterility Assurance Level) compliance for critical pharmaceutical and biological applications.
• Naturally hydrophilic and compatible with aqueous solutions, cellulose nitrate delivers high flow rates and rapid wet-out without requiring pre-conditioning or surfactant treatment.

Air monitoring and stack emissions sampling demand a filter medium that withstands the thermal and chemical conditions at your collection point — the material properties described above define the operating envelope for your application. For this application, the high protein binding capacity of cellulose nitrate provides the strong surface adsorption that your blotting protocols depend on, immobilizing target molecules efficiently for detection with minimal wash-off during subsequent processing steps. Make the decision for consistency and reliability, ensuring that your laboratory maintains its reputation for high-quality, error-free output.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

40.6psi

Color

white

Diameter

50mm

Extractables

low

Filtration Area

19.63cm²

Flow Rate

60ml (Water)

Grid (Yes/No)

Yes

Material

Cellulose Nitrate (CN)

Maximum Operating Pressure

Holder-dependent

Maximum Operating Temperature (°C)

121°C

Membrane Thickness (µm)

145um

Pack Count

100

pH Range

4-8

Pore Size (µm)

0.45

Sterility

Sterile

Sterilization

Supplied Sterile (Single Use)

Support Material

None

Wettability

Hydrophilic

• Gravimetric Particulate Analysis - Dissolves in organic solvents for complete particle recovery and accurate weight-based quantification.
• Aerosol Analysis - Low tare weight and acid digestibility enable chemical characterization of airborne particulates from air sampling.
• Colony Enumeration - Gridded surface variants facilitate accurate plate counting of captured microorganisms under standard magnification.
• Microscopy Examination - Particles remain on the membrane surface for direct visual inspection, supporting identification and classification.
• Biochemical Assays - High protein-binding capacity effectively immobilizes macromolecules, supporting lateral flow and diagnostic testing.
• Routine Sample Prep - Uniform microporous matrix provides consistent flow rates and reliable particle retention for general aqueous solutions.