Cellulose Nitrate Membrane Circle, Plain, 0.2um Pore Size, 47mm, 100/Pk SKU: 10401312

Cellulose Nitrate (CN) Membrane Filters - 10401312

Designed to deliver reproducibility and confidence across critical sample types, the Whatman 10401312 provides the filtration consistency that method validation demands. Precision membrane geometry balances retention sharpness with sustainable flow rates, minimizing the pressure buildup that leads to premature filter replacement. By design, precision manufacturing ensures that each membrane disc delivers the rated retention, flow rate, and surface consistency that analytical and microbiological methods require.

Material Specifications

• High protein binding capacity makes cellulose nitrate the preferred membrane for Western blotting, dot blotting, and nucleic acid immobilization techniques that rely on efficient surface adsorption.
• Surface retention characteristics allow captured particles and microorganisms to remain on the membrane face, supporting direct microscopic examination and physical recovery of collected material.
• Bulk format reduces per-unit packaging waste and cost, appropriate for routine particulate removal and clarification where aseptic handling is not specified.
• Naturally hydrophilic and compatible with aqueous solutions, cellulose nitrate delivers high flow rates and rapid wet-out without requiring pre-conditioning or surfactant treatment.
• Autoclavable at 121°C without loss of integrity, cellulose nitrate membranes maintain their rated pore structure and dimensional stability through repeated sterilization cycles.

Scaling your method from development to routine production requires membrane consistency at every lot — the documented specifications of these membranes provide the reproducibility needed to validate the filtration step once. To support this, surface retention on cellulose nitrate keeps captured particles and microorganisms on the membrane face where they remain accessible for direct microscopic examination, colony counting, and physical recovery without sectioning or dissolution. Take control of your sample preparation variables and ensure that your data reflects the true nature of your work, rather than the quality of your consumables.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

53.6psi

Color

white

Diameter

47mm

Extractables

low

Filtration Area

17.35cm²

Flow Rate

20ml (Water)

Grid (Yes/No)

No

Material

Cellulose Nitrate (CN)

Maximum Operating Pressure

Holder-dependent

Maximum Operating Temperature (°C)

80°C

Membrane Thickness (µm)

105–140um

Pack Count

100

pH Range

4-8

Pore Size (µm)

0.2

Sterility

Nonsterile

Sterilization

Autoclavable at 121°C for 30 mins

Support Material

None

Wettability

Hydrophilic

• Environmental Compliance - Documented retention characteristics at standard pore ratings support reproducibility in regulated testing programs.
• Water Quality Testing - Narrow pore distribution retains indicator organisms consistently for regulatory compliance in environmental laboratories.
• Microscopy Examination - Particles remain on the membrane surface for direct visual inspection, supporting identification and classification.
• Biochemical Assays - High protein-binding capacity effectively immobilizes macromolecules, supporting lateral flow and diagnostic testing.
• Routine Sample Prep - Uniform microporous matrix provides consistent flow rates and reliable particle retention for general aqueous solutions.
• Colony Enumeration - Gridded surface variants facilitate accurate plate counting of captured microorganisms under standard magnification.