NC45 0.45um 90mm 50/Pk SKU: 10401118

Cellulose Nitrate (CN) Membrane Filters - 10401118

The Whatman 10401118 combines a controlled pore size distribution with low extractable levels, ensuring the data accuracy that sensitive analytical methods require. Verified 0.45 pore distribution across the Hydrophilic membrane surface guarantees batch-to-batch consistency in particle capture and analyte passage. Precision manufacturing ensures that each membrane disc delivers the rated retention, flow rate, and surface consistency that analytical and microbiological methods require.

Material Specifications

• Autoclavable at 121°C without loss of integrity, cellulose nitrate membranes maintain their rated pore structure and dimensional stability through repeated sterilization cycles.
• High protein binding capacity makes cellulose nitrate the preferred membrane for Western blotting, dot blotting, and nucleic acid immobilization techniques that rely on efficient surface adsorption.
• Surface retention characteristics allow captured particles and microorganisms to remain on the membrane face, supporting direct microscopic examination and physical recovery of collected material.
• Naturally hydrophilic and compatible with aqueous solutions, cellulose nitrate delivers high flow rates and rapid wet-out without requiring pre-conditioning or surfactant treatment.
• Dissolves completely in specific organic solvents, enabling full recovery of captured particles from the membrane surface for downstream gravimetric, chemical, or spectroscopic analysis.

Whether your method calls for gravimetric analysis, particle visualization, or microbial enumeration, the pore consistency and surface uniformity documented above ensure that your results reflect the sample — not the filter. Because cellulose nitrate dissolves completely in organic solvents like acetone and amyl acetate, you can recover every captured particle from the membrane surface for downstream gravimetric, spectroscopic, or chemical analysis. Make the decision for consistency and reliability, ensuring that your laboratory maintains its reputation for high-quality, error-free output.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

40.6psi

Color

white

Diameter

90mm

Extractables

low

Filtration Area

63.62cm²

Flow Rate

60ml (Water)

Grid (Yes/No)

No

Material

Cellulose Nitrate (CN)

Maximum Operating Pressure

Holder-dependent

Maximum Operating Temperature (°C)

80°C

Membrane Thickness (µm)

105–140um

Pack Count

50

pH Range

4-8

Pore Size (µm)

0.45

Sterility

Nonsterile

Sterilization

Autoclavable at 121°C for 30 mins

Support Material

None

Wettability

Hydrophilic

• Environmental Compliance - Documented retention characteristics at standard pore ratings support reproducibility in regulated testing programs.
• Routine Sample Prep - Uniform microporous matrix provides consistent flow rates and reliable particle retention for general aqueous solutions.
• Colony Enumeration - Gridded surface variants facilitate accurate plate counting of captured microorganisms under standard magnification.
• Bioburden Monitoring - Surface retention keeps captured organisms accessible for direct incubation and enumeration without transfer steps.
• Microscopy Examination - Particles remain on the membrane surface for direct visual inspection, supporting identification and classification.
• Biochemical Assays - High protein-binding capacity effectively immobilizes macromolecules, supporting lateral flow and diagnostic testing.