PROTRAN BA85 82mm 50/Pk SKU: 10401116

Protran Nitrocellulose Blotting Membranes - 10401116

Laboratories running daily blotting protocols require transfer membranes that deliver reproducible signal-to-noise performance from the first circle in the pack to the last. Hydrophilic surface properties allow direct wetting in transfer buffer without methanol activation, preserving native protein conformation for enzymes and other activity-sensitive targets. Reliable transfer performance delivers the binding efficiency and signal clarity needed for confident data interpretation in quantitative applications.

Membrane Specifications

• Pure nitrocellulose construction gives Protran membranes the surface properties needed for superior signal-to-noise ratios without stringent washing conditions, while a proprietary formula preserves bound protein recognition activity for up to five years.
• Proprietary nitrocellulose formula preserves bound protein molecular recognition activity for up to five years of archival storage.
• Protran transfer membranes offer the binding capacity and low background performance that molecular biology laboratories require for routine immunoblotting, dot and slot blotting, and colony and plaque lift applications.
• Neutral membrane surface binds proteins through hydrophobic interactions, providing the low nonspecific background essential for clean immunodetection results.
• Engineered for Western, Southern, and Northern blotting, Protran membranes combine low background fluorescence with broad detection compatibility spanning isotopic, chemiluminescent, colorimetric, and fluorescent methods.

When your research timeline depends on getting defensible blotting data without repeated optimization cycles, the transfer efficiency and signal clarity built into this membrane help you move from experiment to answer with confidence. Pure nitrocellulose formulation with less than 1.0% extractables meets the stringent purity requirements of pharmaceutical protein characterization and clinical diagnostic development. Sample quantities for method validation and compatibility testing are available through Tisch Scientific — request a sample to verify performance in your specific workflow before committing to a production volume.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Diameter

82mm

Material

Nitrocellulose (NC)

Sterility

Nonsterile

Wettability

Hydrophilic

• Protein Immunodetection — Chemiluminescent, colorimetric, and fluorescent visualization of antibody-bound targets for expression studies.
• Colony and Plaque Lifts — In situ transfer of bacterial colonies or phage plaques for hybridization-based screening of genomic and cDNA libraries.
• Northern Blotting — Transfer and hybridization of size-fractionated RNA for gene expression profiling and transcript size determination.
• Lateral Flow Assay Development — Nitrocellulose test strip fabrication for point-of-care diagnostics requiring stable protein immobilization.
• Dot and Slot Blotting — Direct application of protein or nucleic acid samples for rapid semi-quantitative screening without gel separation.
• DNA-Protein Interaction Studies — Immobilization of protein targets for Southwestern blotting and DNA-binding domain characterization experiments.